I-Fluorescence In Hybridation Situation
I-Fluorescence in hybridization (FISH) enye yeendlela zobugcisa ezisetyenziselwa ukukhangela i-DNA yeeseli, ukukhangela ubukho okanye ukungabikho kwamagciwane athile okanye iinxalenye zegesi.
Zininzi iintlobo zesifo somhlaza zidibaniswa nokungaqhelekanga kwemvelo. Kwaye ngemfuza, asikho nje ukuthetha ngokuzalwa. Ngethuba lokuphila, iiseli zenza iimpazamo xa zahlula kwaye zikhula.
Utshintsho kwi-DNA enxulumene nomhlaza lunokuqokelela kule seli.
I-FISH Isebenza njani?
I-FISH iyinkqubo esebenzisa iiproorescent probes ukufumana iizakhi ezithile okanye izitho zegesi (ukulandelelana kweDNA). Abasebenzi bamaziko ebhabhi bezonyango kunye ne- oncologists basebenzisa i-FISH ukunceda ukuhlola izigulane ezinokuba nomhlaza, kwaye ngamanye amaxesha ukujonga isigulane esele safumanisa ukuba unomdlavuza kwaye uphathwa.
I-FISH inokwenziwa ngokusebenzisa iindidi ezahlukeneyo zeesampuli ngokubhekiselele kwindawo kunye nomhlobo womhlaza okhankanyweyo: Iifom ye-tumor ezifunyenwe kwigazi le-peripheral, kwi-bone marrow biopsy okanye kwi-lymph node biopsy, kunye ne-formalin egxininisiweyo ye-tissue (eg isampula yamathambo eqhutywe kwibhuyibhile kwaye ifakwe kwi-wax, eyenza ibe nzima ngakumbi, ukwenzela ukuba ityunyiwe ibe ngamacandelo amancinci kwaye ifakwe ukujonga ngaphantsi kwe-microscope).
Zithini Iincwadi?
"H" kwi-FISH ibhekisela ekungcoleni.
Kwi-hybridation molecule, i-DNA ebhalwe ngegama okanye i-RNA ngokulandelelana isetyenziswe njengeprojekti - bonise itekisi ebomvu ye-Lego, ukuba uyathanda. Inkqubo isetyenziselwa ukufumana umlingani wezitena ze-Lego, okanye i-DNA ngokulandelelana, kwisampula ye-biological.
I-DNA kwi-specimen yakho ifana neenqwaba zezitena ze-Lego, kwaye ezininzi izitena kule mibhobho ayiyi kufana nomzobo wethu obomvu.
Yaye zonke izitena zakho zihlelwe ngokufanelekileyo zibe zibini zombini zezitena zezitena - nganye inqwaba enye yeekromosomes zakho ezinama-homologous ezibiniweyo, ngaphezulu okanye ngaphantsi. Ngokungafani nezitena ze-Lego, iphendi yethu ebomvu ye-Lego ifana nombane onamandla kwaye ufumanisa umdlalo ngaphandle kokuba uhlalutye.
"F" ibhekisela kwi-fluorescence. I-probe yethu ebomvu ingahle ilahleke kwiimfumba zezitena, ngoko ibhalwe ngedayi ye-fluorescent enemibala ukuze ikhanyise. Xa ithola umdlalo phakathi kweepilisi ezingama-23 ezibiniweyo, iteksi ye-fluorescent ibonisa indawo yayo. Ngoko, ngoku unokwazi ukubona indlela abaphandi kunye neekliniki abangasebenzisa ngayo i-FISH ukunceda ukuba (yintoni inqwaba, okanye i-chromosome) i-gene ekhethekileyo.
"I" kunye ne "S" imele kwindawo . Oku kubhekisela ekubeni isibalo sethu sibomvu sibombile umdlalo wakhe kwisampuli owanike yona .
Kuthiwani Ngeengqungquthela Zegazi Zegazi?
I-FISH kunye nezinye iinkqubo zokuxhatshazwa kwe- in situ zisetyenziselwa ukuxilonga iintlobo ezahlukeneyo ze-chromosomal-iinguqu kwimveliso yezofuzo, utshintsho kuma-chromosomes, kuquka oku okulandelayo:
Ukususwa - inxalenye ye-chromosome ihambe
Ukutshintshiselwa - inxalenye yekromosome ikhefu kwaye iyanamathela kwenye i-chromosome
Inversion - inxalenye ye-chromosome ikhefu kwaye ibuyisela kwakhona kwakhona, kodwa ngokulandelelana
Ukuphindaphinda - inxalenye ye-chromosome ikhoyo kwiikopi ezininzi ngaphakathi kweseli
Uhlobo ngalunye lomhlaza lunokuthi lube nolwahlulo lwalo lwe-chromosomal kunye neephes ezifanelekileyo. I-FISH ayinceda kuphela ukuchonga utshintsho lokuqala lwezesifo kwisifo sesifo njengomhlaza, kodwa ingasetyenziselwa ukubeka esweni ukuphendula kwonyango kunye nokuxolelwa kwezifo.
Utshintsho lwezofuzo olufunyenwe ngamaFISH ngamanye amaxesha linikezela ngolwazi olongezelelweyo malunga nendlela umhlaza womntu onokuzenza ngayo, ngokusekelwe kwinto ebonwe ngaphambili kwiindawo ezinomdla ofanayo kunye nohlobo olufanayo lwezofuzo. Ngamanye amaxesha i-FISH isetyenziselwa emva kokuba i-diagnostic sele yenziwe, ukuqokelela ulwazi olongezelelweyo olunokukunceda ukuqikelela isiphumo sesigulane okanye unyango olungcono.
I-FISH iyakwazi ukubona i- chromosomal engafanelekanga kwi-leukemi , kubandakanywa ne-lymphocytic leukemia (CLL) engapheliyo. I-lymphocytic leukemia / i-lymphocytic lymphoma encinci, i-FISH ivumela izigulane ukuba zifumane isigaba sabo sokuxela: esihle, siphezulu okanye sihluphekile. Kwi-acute lymphoblastic leukemia (YONKE), i-genetics yamaseli ekhuhlane anokukuxelela ngengozi yomhlaza kwaye uncede ukukhokela izigqibo zonyango.
Iipaneli ze-FISH nazo ziyafumaneka kwi-lymphoma, i-myeloma eninzi, i-plasma yesistim ephazamisayo kunye ne-myelodysplastic syndrome. Kwimeko yesikhumba se-lymphoma, umzekelo, kukho ukuhanjiswa kwe- FISH okubizwa ngokuba yi-GH / CCND1 t (11; 14) edlalwa rhoqo nale lymphoma.
Kutheni iFISH?
Inzuzo ye-FISH kukuba ayifanele ukuba yenziwe kwiiseli ezihlula ngokukhawuleza. Ukuvavanywa kwe-cytogenetic kuthatha malunga neveki ezintathu, kuba iiseli zomhlaza kufuneka zikhule kwiibhati zeebhabhi malunga neveki ezimbini ngaphambi kokuba zivavanywe. Ngokwahlukileyo, iziphumo ze-FISH zivame ukufumaneka kwilebhu ngaphakathi kweentsuku ezimbalwa.
> Imithombo:
> Uvavanyo lweCrogenetic Testing CPT.
> I-Fluorescence In Hybridization Status (I-FISH). http://www.genome.gov/10000206#al-2.
> I-Fluorescence In Hybridization Status (I-FISH). http://www.nature.com/scitable/topicpage/fluorescence-in-situ-hybridization-fish-327.
> Wolff DJ, Bagg A, Cooley LD, et al. Isikhokelo soFluorescence kwi-Situation Hybridation Testing in Hematologic Disorders. I-Journal ye-Molecular Diagnostics: i-JMD. 2007; 9 (2): 134-143.